Not intended for human or animal diagnostic or therapeutic uses. Phototope ®-HRP Western Blot Detection System: (7071 anti-rabbit) or (7072 anti-mouse) Includes biotinylated protein ladder, secondary (7074 anti-rabbit) or (7076 anti-mouse) antibody conjugated to horseradish peroxidase (HRP), anti-biotin antibody conjugated to HRP, LumiGLO chemiluminescent reagent and peroxide. After transferring the protein bands onto the blot paper, stain the paper with Ponceau-S. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. If you want to do with unstained ladder yet, I recommend to do all steps of western blot just routinely do. Under suggested conditions, Unveil Unstained Protein Ladder resolves 12 major bands in 4-12%Bis- Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane. The Penn State ladder containing 10, 15, 20, 30, 40, 50, 60, 80 100 kD proteins are shown in each panel at a) 1:20, 1:40 and 1:100 dilution in. The ladder is supplied in the gel loading buffer and is ready to use.Ģ0 mM Tris-phosphate(pH 7.5 at 25☌), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol. Use of Penn State protein ladder in Western blots. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |